ABSTRACT
In this paper, we described that using wistar rat rather than Lewis rat, an animal model ofexperimental autoimmune encephalomyelitis (EAE) was established. 12 out of 17 rats showedtypical clinical syndroms and pathological changes, and also developed significant delayed-typeskin hypersen-sitivity reaction to the pathogenic antigen-homogenate of guinea pig spinal cord.The cytotoxicity assay demonstrated that the T-cells of EAE rats can specifically lyse the braintissue of normal rats with specific release rate of 15.1l?t 2.79% which is positive correlativewith clinical severity of EAE. The T cell vaccine was prepared by activating EAE T-cells withConA and then treating with glutaraldehyde. Normal rats were inoculated with this T cell vac-cine and challenged with pathogenic antigen one week later. The result showed that all seven ratsgot complete resistance to EAE. T cells from vaccinated animals can specifically lyse the EAE Tcells with specific release rate of 9.38?1.68%.These results indicated: (a)wistar rat can be used for EAE animal model; (b)The incidence ofEAE is associa ted with the auto-reactive T cellsof EAE animals; (c)Inocula tion of this T cellvaccines can prevent the development of EAE, and this protective effect is also mediated by Tcells.
ABSTRACT
Splenocytes from BALB/c mice immunized with human chorionic gonadotropin(hCG) were fused with myeloma cells NS-1, and 7 hybridomas producing monoclonal antibodies to hCG were established. By using RIA to measure the binding characteristics of these monoclonal antibodies to ~(125)I-labelled hCG, their subunits, and 4 glycoprotein pitiutary hormones, 7 antibodies had been devided into 3 groups. Of these groups one(4 antibodies included) recognized intact hCG and ?-subunit, a second(one antibody only) recognized intact hCG and ?-subunit, and the third(2 antibodies included) bound to the hCG molecule only, The precipitation after double diffusion in agar with hCG antigen demonstrated that 4 antibodies of group 1 recognized 3 epitopes, and 2 antibodies of group 3 recognized different epitopes. The cooperation between pairs of antibodies specific to different epitopes in binding hCG antigen showed that most of these antibodies cooperated each other. The antibody No 10 was showed to be the best, cooperating with every other antibody,where as antibody No 22 was cooperated only with a few antibodies.The coorperation was related to the differency of epitopes recognized, but seemed not to bear relationship with the titre of antibodies.
ABSTRACT
A hybridoma cell line(B10)was established by fusing spleen cells of BALB/c mou- se immunized with human thyroglobulin(HTG) with SP2/0 myeloma cells. An averagefusing rate of 6.3% and antibody-secreting positive well rate of 58.3% were obtained.During the first two months, the supernatant of B10 culture had a titer of 1/128 to1/2048 measured by hemagglutination method, and the ascites was positive at 1/64000-128000 and 1/320000 respectively as measured by hemagglutination and radioimmunoass-ay.The B10 cell line is very stable and has very high activity to produce anti-HTGmonoclonal antibodies. After several times of preservation in liquid nitrogen andpassage in culture for one year,a recent determination shows that cell culture super-natant and ascites still have very high titer,being 1/4096 and 1/1048576 respectively asmeasured by hemagglutination method. The chromosome number of the B10 hybridomacell is 99.5?7.4.The success of the establishment of this cell line is briefly discussed.Attempt to establish diagnostic kit with this monoclonal antibody is being undertaken.
ABSTRACT
Objective:To investigate the effect of hCG on the gene expression of cytokines(TNF??IL 1 and IL 6) that initiate inflammatory reaction protected against bacteria.Methods:Human PBMC were cultured with LPS(22 ?g/ml) and different doses of hCG(100,50,25,12 5,6 25,3 125 U/ml) at 37℃,5%CO 2 for 4 h,mRNA of cells harvested were isolated,then run RT PCR with primer of TNF??IL 1 and IL 6 separately.The PCR product were quantitated by using MIAS 2000 analyzer,calculated the OD value.Results:Compared with the control group,the result showed that hCG in a dose range of 50～6.25 U/ml have significantly suppressive effect on TNF? mRNA expression ( P